Review on the Use of Microwire for Induction of Focal Cerebral Ischemia in Rat

  • Siamak Sandoghchian Shotorbani Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Seyyed Mehdi Vatandoust Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Hajar Ogbaei Department of physiology, Tabriz University of Medical Sciences, Tabriz, Iran
  • Sara Razzaghi Faculty of Veterinary Medicine, Islamic Azad University, Tabriz, Iran
  • Javad Mahmoudi Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

Within the last four decades, attempts have been made to develop reliable preclinical models of ischemic stroke with the aim of discovering its underlying mechanisms and developing novel therapeutic approaches. The middle cerebral artery occlusion (MCAo) technique in rats is able to replicate ischemic stroke with clinical relevance. Induction of MCAo through transfemoral approach using microwire permits researchers to occlude the middle cerebral artery more exactly and safely. Therefore, utilization of this technique reduces animal’s morbidity and mortality, maximizes animal welfare, and minimizes the number of animals required for a study. This article aims to provide a brief overview of problems associating with the induction of MCAo by conventional methods and proposing solutions based on using microwire under fluoroscopic view in the rat.

Published
2020-01-27
Section
Review Article