Effect of Intrahippocampal Injection of Dihydrotestosteron on Spatial Memory in Kindled Male Rats

  • Shirin Babri Neuroscience Research Centre (NSRC), Tabriz University of Medical Sciences, Tabriz
  • Roshanak Ghotbi Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Samira Razavi Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Fariba Mirzaee Bavil Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz,

Abstract

Objective: Dihydrotestosterone (DHT) is an endogenous hormone, derived from testosterone in the enzymatic reaction mediated by 5-alpha reductase. The high density of androgen receptors and 5-alpha reductase enzyme has been found in the hippocampus. According to the recent studies, androgens may have an improving effect on spatial memory. On the other hand, kindling results in learning and memory impairment in rats. In this study, we investigated the effect of intrahippocampal DHT on spatial memory in male kindled rats.

Methods and Materials: 160 male rats randomly divided into 20 groups. (n=8). Control, shame, saline (0.5 µl intrahippocampal), DMSO (0.5 µl intrahippocampal), kindled, DHT (5, 10, 20μg: 30 min or 24 h before test), kindled + DHT (5, 10, 20 μg: 30 min before or after stimulation and then test was done), kindled + DHT (5, 10, 20 μg: injection 30 min before the test). Two canules were implanted in CA1 region of animals in all groups except control group. DHT was solved in 0.5µl DMSO acquisition and retrievals were assessed by Morris Water Maze.

Results: Our findings showed that DHT in doses of 10 and 20 micrograms can improve acquisition and retrieval in kindled male rats. DHT doesn’t have any effect on seizure parameters.

Conclusion: Intrahippocampal DHT can improve acquisition and retrieval in kindled rats but it doesn’t have any effect on seizure parameters.

Published
2017-01-31
Section
Original Article