Effect of Chronic Stress on Coagulation Parameters in Warfarin-Treated Male Rats

stress & coagulation in warfarin-treated rats

  • Mehdi Farhoudi Neurosciences Research Center (NSRC), Department of Neurology, Imam Reza Medical Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Sanaz Foroutan Rad Neurosciences Research Center (NSRC), Imam Reza Medical Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Javad Mahmoudi Neurosciences Research Center (NSRC), Imam Reza Medical Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Saeed Sadigh-Eteghad Neurosciences Research Center (NSRC), Imam Reza Medical Center, Tabriz University of Medical Sciences, Tabriz, Iran
  • Mehrdad Roozbeh Brain Mapping Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Ravinder Jeet Singh Calgary Stroke Program, Cumming School of Medicine, University of Calgary, Calgary, Canada
  • Fatemeh Ebrahimi School of Traditional Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

Objective: Chronic stress affects coagulation system, thus, can potentially interact with oral anticoagulation therapy. Clinically, it can be difficult to induce or quantify stress in human subjects. Therefore, this study proposed to evaluate the effects of chronic stress on coagulation parameters in warfarin-treated rats under controlled conditions.

Materials and Method: Forty male Wistar rats were divided into unstressed and stressed groups. Rats in unstressed group were assigned to control and normal plus warfarin treatment (N-WT) subgroups (n = 10 each). Rats in stressed group underwent chronic mild stress (CMS) and were assigned to CMS and CMS plus warfarin treatment (CMS-WT) subgroups (n = 10 each).

On the 28th day and after completion of the CMS induction procedure, serum corticosterone was used to confirm CMS induction. Also, the coagulation parameters-prothrombin time (PT), partial prothrombin time (PTT), International normalized ratio (INR) and prothrombinlevels-were assessed by biochemical methods

Results: Comparison of changes in PT, PTT and INR values between two groups of N-WT and CMS-WT showed significant increase in PT, PTT and INR values in the CMS-WT(PT:6.6 ± 3.23, p<0.001; PTT: 4.4 ± 3.8, p=0.01 and INR: 0.51 ± 0.23, p<0.001).Prothrombin was significantly higher in CMS group that shows hypercoagulable state. Prothrombin was significantly lower in CMS-WT group which can be due to reduced metabolism of warfarin in this group (0.54 ± 0.017,p<0.001).

Conclusion: CMS decreases prothrombin levels and leads to an increase in measures of timed coagulation parameters (PT, PTT and INR), but in no warfarin treated rats leads to hypercoagulable state.

Author Biography

Mehdi Farhoudi, Neurosciences Research Center (NSRC), Department of Neurology, Imam Reza Medical Center, Tabriz University of Medical Sciences, Tabriz, Iran

Published
2020-01-04
Section
Original Article